BIOL-1415 Introduction to Biotechnology II
iology course that focuses on an integrative approach to studying biomolecules with an emphasis on protein structures, functions and uses in the modern bioscience laboratory. Students will investigate the mechanisms involved in the transfer of information from DNA sequences to proteins to biochemical functions. The course will integrate biological and chemical concepts with techniques that are used in research and industry. Critical thinking will be applied in laboratory exercises using inquiry-based approaches, troubleshooting, and analyzing experimental data. Lec 3, Lab 4, Cr 4
Outcomes
- Demonstrate appropriate laboratory calculations and ability to solve problems involving conversions between unit expressions and the preparation of various solutions, including buffers, to make dilutions and to accurately measure and transfer solutions volumetrically.
- Explain and demonstrate isolation procedures for DNA and proteins by exploiting the various molecular characteristics of these molecules such as size, charge, isoelectric point and hydrophobicity, and demonstrate a basic understanding of bioinformatics by interpreting results.
- Explain and demonstrate a mastery of the fundamentals of column chromatography, including ion exchange and gel filtration, by performing separations of mixtures utilizing low pressure column chromatography.
- Explain and demonstrate a mastery of spectrochemistry, pH meters, and other laboratory equipment used to isolate, purify, and quantitate biomolecules.
- Demonstrate understanding of and ability to carry out proper laboratory procedures within a regulated environment, including maintaining an accurate, up-to-date, and complete notebook record of all research activities.