BIOL-1414 Introduction to Biotechnology I

Overview of classical genetics, DNA structure, the flow of genetic information, DNA replication, gene transcription, protein translation. Principles of major molecular biology and genetic engineering techniques, including restriction enzymes and their uses, major types of cloning vectors, construction of libraries, Southern and Northern blotting, hybridization, PCR, DNA typing. Applications of these techniques in human health and welfare, medicine, agriculture and the environment. Introduction to the human genome project, gene therapy, molecular diagnostics, forensics, creation and uses of transgenic plants and animal and animal cloning and of the ethical, legal, and social issues and scientific problems associated with these technologies. Relevant practical exercises in the above areas. Lec 3, Lab 4, Cr 4

Credits

4

Prerequisite

ENGL-1301; Minimum grade C, TSI Ready in Mathematics

Outcomes

  1. Demonstrate, both verbally and in writing, knowledge of the field of biotechnology and applications of genomics, including historical development of the field.
  2. Demonstrate, both verbally and in writing, knowledge of scientific theory related to biotechnology techniques.
  3. Utilizing the S.I. system of units, properly and safely operate a variety of laboratory tools and equipment to learn about and develop basic lab skills such as pipetting, preparing solutions, and weighing, and measuring.
  4. Demonstrate, both verbally and in writing, knowledge of DNA, RNA, and protein structures, including their connection to classical genetics and the flow of genetic information.
  5. Demonstrate understanding of and ability to utilize proper laboratory procedures within a regulated environment, including maintaining an accurate, up-to-date, and complete notebook record of all research activities.
  6. Consider, discuss and debate current ethical and legal issues in biotechnology.